Microscopy

I excel in fluorescence microscopy techniques, from confocal to wide-field microscopy, with a particular emphasis on quantitative methods such as

• Localization microscopy
• FRAP (Fluorescence Recovery After Photobleaching) microscopy
• TIRF (Total Internal Reflection Fluorescence) microscopy
• Differential evanescence field nanometry (Merrifield, et al., 2002; Merrifield, et al., 2005, Saffarian, et al., 2008)
• Microscopy automation and computer vision for high throughput of rare events.

I have been using all these techniques to quantify or interfere with transient microscopic events of cellular biology for over a decade.

I can assist you with everything from planning such experiments, such as sample preparation, genomic engineering, or the optimization of the microscope light path, to developing dedicated software or executing the imaging itself.

Differential evanescence field imaging of endocytosis in S. cerevisiae. In green is an endocytic coat marker, Sla1, tagger with EGFP. In magenta  is an actin crosslinker, Sac6, tagged with mCherry. From left to right: bright field image, wide-field movie (WF), and TIRF movies on the green and red channels acquired simultaneously with a Hamamatsu Gemini beam splitter. The disappearance of the green spot in TIRF, but not in WF, marks the beginning of the coat internalisation (Picco, et al., 2024). Movie plays 7x faster.

Optical tickling of F. alba invasive collectives, imaged in bright field (Toret, et al., 2022). Scale bar is 25 μm.